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sgrna transcription kit  (TaKaRa)


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    Structured Review

    TaKaRa sgrna transcription kit
    Sgrna Transcription Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sgrna+in+vitro+transcription+kit/Guide-it+sgRNA+In+Vitro+Transcription+Kit/pm42684483-59-8-11
    Average 95 stars, based on 152 article reviews
    sgrna transcription kit - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Biomarker Discovery:

    Article Title: Targeted Gene Editing in Honey Bees Using Liposome-Based CRISPR-Cas9.
    Article Snippet: Genome editing technologies have revolutionized molecular biology, enabling precise manipulation of gene functions across diverse organisms.. In this study, we introduce a novel liposome-mediated delivery system for CRISPR-Cas9 components targeting the Juvenile Hormone Acid Methyltransferase (JHAMT) gene in honey bees (Apis mellifera anatoliaca).. This approach leverages drone sperm cells as vectors for CRISPR-Cas9 transfection, overcoming the technical challenges of embryo microinjection in honey bees, such as low survival rates and labor-intensive procedures.

    Sequencing:

    Article Title: Targeted Gene Editing in Honey Bees Using Liposome-Based CRISPR-Cas9.
    Article Snippet: Genome editing technologies have revolutionized molecular biology, enabling precise manipulation of gene functions across diverse organisms.. In this study, we introduce a novel liposome-mediated delivery system for CRISPR-Cas9 components targeting the Juvenile Hormone Acid Methyltransferase (JHAMT) gene in honey bees (Apis mellifera anatoliaca).. This approach leverages drone sperm cells as vectors for CRISPR-Cas9 transfection, overcoming the technical challenges of embryo microinjection in honey bees, such as low survival rates and labor-intensive procedures.

    Article Title: A Single-Cell Transcriptomic Atlas of Symmetry Breaking Across Eutherian Mammals
    Article Snippet: .. The sgRNA (CTTGCACGCC TGGTGG GCTC) was produced using the Guide-it sgRNA in vitro Transcription kit® (Takara Bio, USA) using a primer containing a T7 promoter and the sgRNA sequence ( Supplementary Table 1 ). .. Capped polyadenylated BE3-encoding mRNA was produced by in vitro transcription using the mMESSAGE mMACHINE T7 ULTRA kit (Life Technologies) and the plasmid pCMV-BE3 (Addgene #73021) linearized with BbsI. mRNA was purified using the MEGAClear kit (Life Technologies).

    Cleavage Assay:

    Article Title: Targeted Gene Editing in Honey Bees Using Liposome-Based CRISPR-Cas9.
    Article Snippet: Genome editing technologies have revolutionized molecular biology, enabling precise manipulation of gene functions across diverse organisms.. In this study, we introduce a novel liposome-mediated delivery system for CRISPR-Cas9 components targeting the Juvenile Hormone Acid Methyltransferase (JHAMT) gene in honey bees (Apis mellifera anatoliaca).. This approach leverages drone sperm cells as vectors for CRISPR-Cas9 transfection, overcoming the technical challenges of embryo microinjection in honey bees, such as low survival rates and labor-intensive procedures.

    In Silico:

    Article Title: Targeted Gene Editing in Honey Bees Using Liposome-Based CRISPR-Cas9.
    Article Snippet: Genome editing technologies have revolutionized molecular biology, enabling precise manipulation of gene functions across diverse organisms.. In this study, we introduce a novel liposome-mediated delivery system for CRISPR-Cas9 components targeting the Juvenile Hormone Acid Methyltransferase (JHAMT) gene in honey bees (Apis mellifera anatoliaca).. This approach leverages drone sperm cells as vectors for CRISPR-Cas9 transfection, overcoming the technical challenges of embryo microinjection in honey bees, such as low survival rates and labor-intensive procedures.

    Generated:

    Article Title: Targeted Gene Editing in Honey Bees Using Liposome-Based CRISPR-Cas9.
    Article Snippet: Genome editing technologies have revolutionized molecular biology, enabling precise manipulation of gene functions across diverse organisms.. In this study, we introduce a novel liposome-mediated delivery system for CRISPR-Cas9 components targeting the Juvenile Hormone Acid Methyltransferase (JHAMT) gene in honey bees (Apis mellifera anatoliaca).. This approach leverages drone sperm cells as vectors for CRISPR-Cas9 transfection, overcoming the technical challenges of embryo microinjection in honey bees, such as low survival rates and labor-intensive procedures.

    Polymerase Chain Reaction:

    Article Title: Targeted Gene Editing in Honey Bees Using Liposome-Based CRISPR-Cas9.
    Article Snippet: Genome editing technologies have revolutionized molecular biology, enabling precise manipulation of gene functions across diverse organisms.. In this study, we introduce a novel liposome-mediated delivery system for CRISPR-Cas9 components targeting the Juvenile Hormone Acid Methyltransferase (JHAMT) gene in honey bees (Apis mellifera anatoliaca).. This approach leverages drone sperm cells as vectors for CRISPR-Cas9 transfection, overcoming the technical challenges of embryo microinjection in honey bees, such as low survival rates and labor-intensive procedures.

    In Vitro:

    Article Title: Targeted Gene Editing in Honey Bees Using Liposome-Based CRISPR-Cas9.
    Article Snippet: Genome editing technologies have revolutionized molecular biology, enabling precise manipulation of gene functions across diverse organisms.. In this study, we introduce a novel liposome-mediated delivery system for CRISPR-Cas9 components targeting the Juvenile Hormone Acid Methyltransferase (JHAMT) gene in honey bees (Apis mellifera anatoliaca).. This approach leverages drone sperm cells as vectors for CRISPR-Cas9 transfection, overcoming the technical challenges of embryo microinjection in honey bees, such as low survival rates and labor-intensive procedures.

    Article Title: Generation of a Syngeneic Heterozygous ACVRL1 (wt/mut) Knockout iPS Cell Line for the In Vitro Study of HHT2-Associated Angiogenesis.
    Article Snippet: .. The synthesis of sgRNAs was performed with the Guide-it sgRNA in vitro transcription kit and Guide-it IVT RNA clean-up kit (Takara Bio Inc., Shiga, Japan) according to the manufacturer’s protocol. .. Cas9-dependent in vitro cutting efficiency was evaluated with the Guide-it sgRNA screening kit (Takara Bio Inc.).

    Article Title: Generation of a Syngeneic Heterozygous ACVRL1 (wt/mut) Knockout iPS Cell Line for the In Vitro Study of HHT2-Associated Angiogenesis
    Article Snippet: .. The synthesis of sgRNAs was performed with the Guide-it sgRNA in vitro transcription kit and Guide-it IVT RNA clean-up kit (Takara Bio Inc., Shiga, Japan) according to the manufacturer’s protocol. .. Cas9-dependent in vitro cutting efficiency was evaluated with the Guide-it sgRNA screening kit (Takara Bio Inc.).

    Article Title: Improvement of fatty acid productivity of thraustochytrid, Aurantiochytrium sp. by genome editing.
    Article Snippet: 1389-1723/$ https://doi.or Thraustochytrid strains belonging to the genus Aurantiochytrium accumulate significant amounts of lipids including polyunsaturated fatty acids and carotenoids and, therefore, are expected to be used for industrial production of various valuable materials.. Although various efforts such as chemical mutagenesis and homologous gene recombination have been made to improve lipid productivity of Aurantiochytrium species, low specificity and efficiency in the conventional methods hinder the research progress.. Here, we attempted to apply a genome editing technology, the CRISPR-Cas9 system as an alternative molecular breeding technique for Aurantiochytrium species to accelerate the metabolic engineering.

    Article Title: Post-transcriptional repression of circadian component CLOCK regulates cancer-stemness in murine breast cancer cells
    Article Snippet: Commercial assay or kit , Taqman MicroRNA Assay , Applied Biosystems , Cat #: 4427975 , . .. Commercial assay or kit , Guide-it sgRNA in vitro transcription kit , Clontech , Cat #: Z2635N , . .. Commercial assay or kit , Lentiviral High Titer Packaging Mix with pLVSIN series , Takara bio , Cat #: 6952 , .

    Article Title: Effect of Bevacizumab on a Human Breast Cancer Model that Exhibited Palbociclib-resistance by RB Knockout
    Article Snippet: .. Single guide RNA (sgRNA) was synthesized using the Guide-it sgRNA in vitro transcription kit (Takara Bio, Siga, Japan). ..

    Article Title: A Single-Cell Transcriptomic Atlas of Symmetry Breaking Across Eutherian Mammals
    Article Snippet: .. The sgRNA (CTTGCACGCC TGGTGG GCTC) was produced using the Guide-it sgRNA in vitro Transcription kit® (Takara Bio, USA) using a primer containing a T7 promoter and the sgRNA sequence ( Supplementary Table 1 ). .. Capped polyadenylated BE3-encoding mRNA was produced by in vitro transcription using the mMESSAGE mMACHINE T7 ULTRA kit (Life Technologies) and the plasmid pCMV-BE3 (Addgene #73021) linearized with BbsI. mRNA was purified using the MEGAClear kit (Life Technologies).

    Article Title: Topologically associating domains define the impact of de novo promoter variants on autism spectrum disorder risk
    Article Snippet: .. The sgRNAs were designed by using CHOPCHOP and synthesized by in vitro transcription (IVT) using Guide-itTM sgRNA In vitro Transcription Kit (Takara Bio Inc.) following the manufacturer's protocol. .. The ssODNs were generated by Fasmac Co., Ltd. (Kanagawa, Japan).

    Synthesized:

    Article Title: Effect of Bevacizumab on a Human Breast Cancer Model that Exhibited Palbociclib-resistance by RB Knockout
    Article Snippet: .. Single guide RNA (sgRNA) was synthesized using the Guide-it sgRNA in vitro transcription kit (Takara Bio, Siga, Japan). ..

    Article Title: Topologically associating domains define the impact of de novo promoter variants on autism spectrum disorder risk
    Article Snippet: .. The sgRNAs were designed by using CHOPCHOP and synthesized by in vitro transcription (IVT) using Guide-itTM sgRNA In vitro Transcription Kit (Takara Bio Inc.) following the manufacturer's protocol. .. The ssODNs were generated by Fasmac Co., Ltd. (Kanagawa, Japan).

    Produced:

    Article Title: A Single-Cell Transcriptomic Atlas of Symmetry Breaking Across Eutherian Mammals
    Article Snippet: .. The sgRNA (CTTGCACGCC TGGTGG GCTC) was produced using the Guide-it sgRNA in vitro Transcription kit® (Takara Bio, USA) using a primer containing a T7 promoter and the sgRNA sequence ( Supplementary Table 1 ). .. Capped polyadenylated BE3-encoding mRNA was produced by in vitro transcription using the mMESSAGE mMACHINE T7 ULTRA kit (Life Technologies) and the plasmid pCMV-BE3 (Addgene #73021) linearized with BbsI. mRNA was purified using the MEGAClear kit (Life Technologies).



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